Recombinant humaan pyridoxaal kinase/PDXK-eiwit

PDXK converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Recombinant human PDXK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.6 kDa (336aa) confirmed by MALDI-TOF

Pyridoxal Kinase, PDXK, Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase, ATGP0777-10 µg, ATGP0777-20 µg, ATGP0777-50 µg, ATGP0777-100 µg, ATGP0777-250 µg, ATGP0777-500 µg, ATGP0777-1 mg, ATGP0777-10, ATGP0777-20, ATGP0777-50, ATGP0777-100, ATGP0777-250, ATGP0777-500, ATGP0777-1

Merrill AH Jr., et al. (1984) J Nutr. 114 (9) :1664-74.; ; Hanna M C., et al. (1997) J Biol Chem. 272 (16) :10756-60.;

Maity AN, et al. The Nitrogen Atom of Vitamin B6 Is Essential for the Catalysis of Radical Aminomutases. (Int J Mol Sci. 2022) {https://pubmed.ncbi.nlm.nih.gov/35563602/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_003672

O00764

Human

MGSSHHHHHH SSGLVPRGSH MGSHMEEECR VLSIQSHVIR GYVGNRAATF PLQVLGFEID AVNSVQFSNH TGYAHWKGQV LNSDELQELY EGLRLNNMNK YDYVLTGYTR DKSFLAMVVD IVQELKQQNP RLVYVCDPVL GDKWDGEGSM YVPEDLLPVY KEKVVPLADI ITPNQFEAEL LSGRKIHSQE EALRVMDMLH SMGPDTVVIT SSDLPSPQGS NYLIVLGSQR RRNPAGSVVM ERIRMDIRKV DAVFVGTGDL FAAMLLAWTH KHPNNLKVAC EKTVSTLHHV LQRTIQCAKA QAGEGVRPSP MQLELRMVQS KRDIEDPEIV VQATVL