Recombinant humaan QDPR-eiwit

QDPR is a member of the short-chain dehydrogenases/reductase (SDR) family of enzymes. Functioning as a homodimer, QDPR plays an important role in the recycling of tetrahydrobiopterin (BH4), an essential cofactor for the hydroxylation of the aromatic amino acids (tryptophan, tyrosine and phenylalanine) . More specifically, QDPR catalyzes the regeneration of BH4 from quinonoid dihydrobiopterin (qBH2), the product generated from the hydroxylation reactions. Mutations in the gene encoding QDPR can lead to phenylketonuria II. Recombinant human QDPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Quinoid dihydropteridine reductase., DHPR, FLJ42391, PKu2, SDR33C1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.2 kDa (267aa) confirmed by MALDI-TOF

QDPR, Quinoid dihydropteridine reductase., DHPR, FLJ42391, PKu2, SDR33C1, ATGP1172-10 µg, ATGP1172-20 µg, ATGP1172-50 µg, ATGP1172-100 µg, ATGP1172-250 µg, ATGP1172-500 µg, ATGP1172-1 mg, ATGP1172-10, ATGP1172-20, ATGP1172-50, ATGP1172-100, ATGP1172-250, ATGP1172-500, ATGP1172-1

Lye LF., et al. (2002) J Biol Chem. 277 (41) :38245-53.; ; Kalkanoqlu HS., et al. (2001) Prenat Diagn. 21 (10) :868-70.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

NP_000311

P09417

Human

MAAAAAA GEARRVLVYG GRGALGSRCV QAFRARNWWV ASVDVVENEE ASASIIVKMT DSFTEQADQV TAEVGKLLGE EKVDAILCVA GGWAGGNAKS KSLFKNCDLM WKQSIWTSTI SSHLATKHLK EGGLLTLAGA KAALDGTPGM IGYGMAKGAV HQLCQSLAGK NSGMPPGAAA IAVLPVTLDT PMNRKSMPEA DFSSWTPLEF LVETFHDWIT GKNRPSSGSL IQVVTTEGRT ELTPAYF