Recombinant humaan QPCT-eiwit

QPCT, also known as glutaminyl-peptide cyclotransferase, is responsible for the biosynthesis of pyroglutamyl peptides. This protein has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. It catalyzes N-terminal pyroglutamate formation. In vitro, it catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. Recombinant human QPCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Glutaminyl-peptide cyclotransferase, GCT, QC, sQC

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

38.7 kDa (339aa)

QPCT, Glutaminyl-peptide cyclotransferase, GCT, QC, sQC, ATGP3153-10 µg, ATGP3153-20 µg, ATGP3153-50 µg, ATGP3153-100 µg, ATGP3153-250 µg, ATGP3153-500 µg, ATGP3153-1 mg, ATGP3153-10, ATGP3153-20, ATGP3153-50, ATGP3153-100, ATGP3153-250, ATGP3153-500, ATGP3153-1

Schilling S. et al., (2004) FEBS Lett. 563:191-196.; ; Huang K.F. et al., (2011) J. Biol. Chem. 286:12439-12449.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_036545

Q16769

Human

VSPSASAWPE EKNYHQPAIL NSSALRQIAE GTSISEMWQN DLQPLLIERY PGSPGSYAAR QHIMQRIQRL QADWVLEIDT FLSQTPYGYR SFSNIISTLN PTAKRHLVLA CHYDSKYFSH WNNRVFVGAT DSAVPCAMML ELARALDKKL LSLKTVSDSK PDLSLQLIFF DGEEAFLHWS PQDSLYGSRH LAAKMASTPH PPGARGTSQL HGMDLLVLLD LIGAPNPTFP NFFPNSARWF ERLQAIEHEL HELGLLKDHS LEGRYFQNYS YGGVIQDDHI PFLRRGVPVL HLIPSPFPEV WHTMDDNEEN LDESTIDNLN KILQVFVLEY LHLHHHHHH