Recombinant humaan QRT1-eiwit

QTRT1 also known as queuine tRNA-ribosyltransferase. QTRT1 interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7- (((4, 5-cis-dihydroxy-2-cyclopenten-1-yl) amino) methyl) -7-deazaguanosine) . Recombinant QTRT1 was expressed in E. coli and purified by using conventional chromatography techniques.

Queuine tRNA-ribosyltransferase, FP3235, TGT, TGUT

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

46.7 kDa (428aa) confirmed by MALDI-TOF

QTRT1, Queuine tRNA-ribosyltransferase, FP3235, TGT, TGUT, ATGP3099-10 µg, ATGP3099-20 µg, ATGP3099-50 µg, ATGP3099-100 µg, ATGP3099-250 µg, ATGP3099-500 µg, ATGP3099-1 mg, ATGP3099-10, ATGP3099-20, ATGP3099-50, ATGP3099-100, ATGP3099-250, ATGP3099-500, ATGP3099-1

Deshpande K.L., et al. (2001) Gene 265:205-212

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_112486

Q9BXR0

Human

MAGAA TQASLESAPR IMRLVAECSR SRARAGELWL PHGTVATPVF MPVGTQATMK GITTEQLDAL GCRICLGNTY HLGLRPGPEL IQKANGLHGF MNWPHNLLTD SGGFQMVSLV SLSEVTEEGV RFRSPYDGNE TLLSPEKSVQ IQNALGSDII MQLDDVVSST VTGPRVEEAM YRSIRWLDRC IAAHQRPDKQ NLFAIIQGGL DADLRATCLE EMTKRDVPGF AIGGLSGGES KSQFWRMVAL STSRLPKDKP RYLMGVGYAT DLVVCVALGC DMFDCVFPTR TARFGSALVP TGNLQLRKKV FEKDFGPIDP ECTCPTCQKH SRAFLHALLH SDNTAALHHL TVHNIAYQLQ LMSAVRTSIV EKRFPDFVRD FMGAMYGDPT LCPTWATDAL ASVGITLG