Recombinant humaan QTRTD1-eiwit

QTRTD1 interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GuN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine. Recombinant human QTRTD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Queuine tRNA-ribosyltransferase subunit QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, FLJ12960, Queuine tRNA-ribosyltransferase domain-containing protein 1, Queuine tRNA ribosyltransferase subunit QTRTD1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

49.1 kDa (438aa) confirmed by MALDI-TOF

QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, Queuine tRNA-ribosyltransferase subunit QTRTD1, FLJ12960, Queuine tRNA-ribosyltransferase domain-containing protein 1, Queuine tRNA ribosyltransferase subunit QTRTD1, ATGP2868-10 µg, ATGP2868-20 µg, ATGP2868-50 µg, ATGP2868-100 µg, ATGP2868-250 µg, ATGP2868-500 µg, ATGP2868-1 mg, ATGP2868-10, ATGP2868-20, ATGP2868-50, ATGP2868-100, ATGP2868-250, ATGP2868-500, ATGP2868-1

Chen, Y.C., et al. (2010) RNA 16 (5), 958-968

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 2mM DTT, 1mM EDTA, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_078914

Q9H974

Human

MGSSHHHHHH SSGLVPRGSH MGSMKLSLTK VVNGCRLGKI KNLGKTGDHT MDIPGCLLYT KTGSAPHLTH HTLHNIHGVP AMAQLTLSSL AEHHEVLTEY KEGVGKFIGM PESLLYCSLH DPVSPCPAGY VTNKSVSVWS VAGRVEMTVS KFMAIQKALQ PDWFQCLSDG EVSCKEATSI KRVRKSVDRS LLFLDNCLRL QEESEVLQKS VIIGVIEGGD VMEERLRSAR ETAKRPVGGF LLDGFQGNPT TLEARLRLLS SVTAELPEDK PRLISGVSRP DEVLECIERG VDLFESFFPY QVTERGCALT FSFDYQPNPE ETLLQQNGTQ EEIKCMDQIK KIETTGCNQE ITSFEINLKE KKYQEDFNPL VRGCSCYCCK NHTRAYIHHL LVTNELLAGV LLMMHNFEHY FGFFHYIREA LKSDKLAQLK ELIHRQAS