Recombinant humaan RAB13-eiwit

RAB13 (Ras-related protein Rab-13) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs) . It is expressed at all stages of preimplantation development. Recombinant human RAB13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Rab-13, GIG4, RAB13 member RAS oncogene family, Cell growth-inhibiting gene 4 protein

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.7 kDa (220aa) confirmed by MALDI-TOF

Rab13, Ras-related protein Rab-13, GIG4, ATGP1126-10 µg, ATGP1126-20 µg, ATGP1126-50 µg, ATGP1126-100 µg, ATGP1126-250 µg, ATGP1126-500 µg, ATGP1126-1 mg, ATGP1126-10, ATGP1126-20, ATGP1126-50, ATGP1126-100, ATGP1126-250, ATGP1126-500, ATGP1126-1

Sheth B., et al. (2000) Mech Dev. 97 (1-2) :93-104.; ; Kohler K., et al. (2004) J Cell Biol. 165 (2) :175-80.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 200 mM NaCl, 1mM EDTA

Signal Transduction

NP_002861.1

P51153

Human

MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC