Recombinant humaan RAB14-eiwit

RAB14 is a member of the RAB protein family, low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane bound organelles. This protein is thought to be involved in vesicular trafficking and neurotransmitter release throughout the body and is expressed at high levels in brain, lung, kidney, spleen and thymus. It is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments. Recombinant human RAB14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ras-related protein Rab-14, FBP, RAB-14, RAB14 member RAS oncogene family, F protein-binding protein 1, bA165P4.3 (member RAS oncogene family), Small GTP binding protein RAB14

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.3 kDa (238aa) confirmed by MALDI-TOF

RAB14, Ras-related protein Rab-14, FBP, RAB-14, ATGP1107-10 µg, ATGP1107-20 µg, ATGP1107-50 µg, ATGP1107-100 µg, ATGP1107-250 µg, ATGP1107-500 µg, ATGP1107-1 mg, ATGP1107-10, ATGP1107-20, ATGP1107-50, ATGP1107-100, ATGP1107-250, ATGP1107-500, ATGP1107-1

Junutula JR. et al. (2004) Mol Biol Cell. 15:2218-29.; ; Olkkonen V.M. et al. (1993) J. Cell Sci. 106:1249-61.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_057406

P61106

Human

MGSSHHHHHH SSGLVPRGSH MGSMATAPYN YSYIFKYIII GDMGVGKSCL LHQFTEKKFM ADCPHTIGVE FGTRIIEVSG QKIKLQIWDT AGQERFRAVT RSYYRGAAGA LMVYDITRRS TYNHLSSWLT DARNLTNPNT VIILIGNKAD LEAQRDVTYE EAKQFAEENG LLFLEASAKT GENVEDAFLE AAKKIYQNIQ DGSLDLNAAE SGVQHKPSAP QGGRLTSEPQ PQREGCGC