Recombinant humaan RAB23-eiwit

RAB23, also known as ras-related protein Rab23, is a member of the Rab family of proteins and localizes to the cytoplasmic side of the cell membrane. This protein is thought to to play a role in intracellular protein transportation and signal transduction mediated by small GTPases. RAB23 is an essential negative regulator of the Sonic hedgehog signaling pathway. Mutations in the gene encoding RAB23 may result in Carpenter syndrome a condition characterized by obesity, cardiac defects, polysyndactyly and craniosynostosis. Recombinant human RAB23 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ras-related protein Rab-23, DKFZp781H0695, HSPC137, MGC8900, RAB23 member RAS oncogene family

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.5 kDa (254aa) confirmed by MALDI-TOF

RAB23, Ras-related protein Rab-23, DKFZp781H0695, HSPC137, MGC8900, ATGP1290-10 µg, ATGP1290-20 µg, ATGP1290-50 µg, ATGP1290-100 µg, ATGP1290-250 µg, ATGP1290-500 µg, ATGP1290-1 mg, ATGP1290-10, ATGP1290-20, ATGP1290-50, ATGP1290-100, ATGP1290-250, ATGP1290-500, ATGP1290-1

Marcos I. et al. (2003) Int J Mol Med. 12:983-987.; ; Eggenschwiler JT. et al. (2001) Nature. 412:194-198.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 1mM DTT, 0.1mM PMSF

Signal Transduction

NP_057361

Q9ULC3

Human

MGSSHHHHHH SSGLVPRGSH MLEEDMEVAI KMVVVGNGAV GKSSMIQRYC KGIFTKDYKK TIGVDFLERQ IQVNDEDVRL MLWDTAGQEE FDAITKAYYR GAQACVLVFS TTDRESFEAV SSWREKVVAE VGDIPTVLVQ NKIDLLDDSC IKNEEAEALA KRLKLRFYRT SVKEDLNVNE VFKYLAEKYL QKLKQQIAED PELTHSSSNK IGVFNTSGGS HSGQNSGTLN GGDVINLRPN KQRTKKNRNP FSSC