Recombinant humaan RAB2A-eiwit

RAB2A (Ras-related protein Rab-2A) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. RAB2A and RAB2B are required for protein transport from the ER to the Golgi, RAB2A is lipid-anchored to the ER-Golgi intermediate compartment membrane while RAB2B is lipid anchored to the cytoplasmic side of the cell membrane. RAB2A has been shown to interact with GOLGA2. Recombinant human RAB2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Rab-2A, RAB2, RAB2A member RAS oncogene family

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.1 kDa (236aa) confirmed by MALDI-TOF

RAB2A, ras-related protein Rab-2A, RAB2, ATGP1098-10 µg, ATGP1098-20 µg, ATGP1098-50 µg, ATGP1098-100 µg, ATGP1098-250 µg, ATGP1098-500 µg, ATGP1098-1 mg, ATGP1098-10, ATGP1098-20, ATGP1098-50, ATGP1098-100, ATGP1098-250, ATGP1098-500, ATGP1098-1

Opdam, F.J., et al. (2000) Eur. J. Cell Biol. 79: 308-316.; ; Tisdale E.J. (2003) J. Biol. Chem. 278:52524-52530

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Signal Transduction

NP_002856.1

P61019

Human

MAYAYL FKYIIIGDTG VGKSCLLLQF TDKRFQPVHD LTIGVEFGAR MITIDGKQIK LQIWDTAGQE SFRSITRSYY RGAAGALLVY DITRRDTFNH LTTWLEDARQ HSNSNMVIML IGNKSDLESR REVKKEEGEA FAREHGLIFM ETSAKTASNV EEAFINTAKE IYEKIQEGVF DINNEANGIK IGPQHAATNA THAGNQGGQQ AGGGCC