Recombinant humaan RAB32-eiwit

RAB32 belongs to the small GTPase superfamily. RAB32 modulates ER calcium handling and disrupts the specific enrichment of calnexin on the MAM (mitochondria-associated membrane), while not affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Also, RAB32 determines the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and through its overexpression or inactivation increases the phosphorylation of Bad and of Drp1. Through a combination of its functions as a PKA-anchoring protein and a regulator of MAM properties, the activity and expression level of RAB32 determine the speed of apoptosis onset. Recombinant human RAB32 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Rab-32, RAB32 member RAS oncogene family

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.5 kDa (249aa) confirmed by MALDI-TOF

RAB32, Ras-related protein Rab-32, ATGP1246-10 µg, ATGP1246-20 µg, ATGP1246-50 µg, ATGP1246-100 µg, ATGP1246-250 µg, ATGP1246-500 µg, ATGP1246-1 mg, ATGP1246-10, ATGP1246-20, ATGP1246-50, ATGP1246-100, ATGP1246-250, ATGP1246-500, ATGP1246-1

Bui M, et al. (2010) J Biol Chem. 285 (41) :31590-602.; ; Alto NM, et al. (2002) J Cell Biol.158 (4) :659-68.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200 mM NaCl, 2mM EDTA

Signal Transduction

NP_006825

Q13637

Human

MAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC