Recombinant humaan RAB34-eiwit

RAB34, also known as ras-related protein Rab34, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. This protein is involved in protein transport, specifically the redistribution of lysosomes to the peri-Golgi region. In fibroblasts, this protein was colocalized with actin to the membrane ruffles and membranes of relatively large vesicles adjacent to the ruffles. Recombinant human RAB34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ras-related protein Rab-34, RAB39, RAH, RAB34 member RAS oncogene family, Ras-related protein Rab-39, Ras-related protein Rah, nine-amino acid residue-repeats, NARR

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.2 kDa (279aa) confirmed by MALDI-TOF

RAB34, Ras-related protein Rab-34, RAB39, RAH, ATGP1291-10 µg, ATGP1291-20 µg, ATGP1291-50 µg, ATGP1291-100 µg, ATGP1291-250 µg, ATGP1291-500 µg, ATGP1291-1 mg, ATGP1291-10, ATGP1291-20, ATGP1291-50, ATGP1291-100, ATGP1291-250, ATGP1291-500, ATGP1291-1

Sun P. et al. (2003) J Biol Chem. 278 :4063-71.; ; Novick P. et al. (1993) Cell. 75: 597-601.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_114140

Q9BZG1

Human

MGSSHHHHHH SSGLVPRGSH MNILAPVRRD RVLAELPQCL RKEAALHGHK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGIP FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLADA LKENDPSSVL LFLVGSKKDL STPAQYALME KDALQVAQEM KAEYWAVSSL TGENVREFFF RVAALTFEAN VLAELEKSGA RRIGDVVRIN SDDSNLYLTA SKKKPTCCP