Recombinant humaan RAB35-eiwit

RAB35, also known as ras-related protein Rab-35, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Consistent with a key requirement for Rab35-regulated recycling during cell division, inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis. Recombinant human RAB35 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ras-related protein Rab-35, H-ray, RAB1C, RAY, RAB35 member RAS oncogene family, GTP-binding protein RAY, Ras-related protein Rab-1C

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.1 kDa (221aa) confirmed by MALDI-TOF

RAB35, Ras-related protein Rab-35, H-ray, RAB1C, RAY, ATGP1218-10 µg, ATGP1218-20 µg, ATGP1218-50 µg, ATGP1218-100 µg, ATGP1218-250 µg, ATGP1218-500 µg, ATGP1218-1 mg, ATGP1218-10, ATGP1218-20, ATGP1218-50, ATGP1218-100, ATGP1218-250, ATGP1218-500, ATGP1218-1

Kouranti I., et al. (2006) Curr. Biol. 16:1719-1725; ; Marat A.L., et al. (2010) J. Biol. Chem. 285:10627-10637

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_006852.1

Q15286

Human

MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C