Recombinant humaan RAB3B-eiwit

RAB3B, member of RAS oncogene family, is a highly homologous monomeric GTPase that may play a role in endocytosis and biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles. Recombinant human RAB3B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Rab-3B, RAB3B member RAS oncogene family, Rab-3B, Rab 3B, RAB3B member RAS oncogene family

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.9 kDa (239aa) confirmed by MALDI-TOF

RAB3B, ATGP1111-10 µg, ATGP1111-20 µg, ATGP1111-50 µg, ATGP1111-100 µg, ATGP1111-250 µg, ATGP1111-500 µg, ATGP1111-1 mg, ATGP1111-10, ATGP1111-20, ATGP1111-50, ATGP1111-100, ATGP1111-250, ATGP1111-500, ATGP1111-1

Zahraoui A., et al. (1989) J Biol Chem. 264 (21) :12394-401.; ; Chung CY., et al. (2009) Proc Natl Acad Sci u S A. 106 (52) :22474-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl

Signal Transduction

NP_002858

P20337

Human

MASVTDGKTG VKDASDQNFD YMFKLLIIGN SSVGKTSFLF RYADDTFTPA FVSTVGIDFK VKTVYRHEKR VKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWATQI KTYSWDNAQV ILVGNKCDME EERVVPTEKG QLLAEQLGFD FFEASAKENI SVRQAFERLV DAICDKMSDS LDTDPSMLGS SKNTRLSDTP PLLQQNCSC