Recombinant humaan RAB3D-eiwit

Ras-related protein Rab-3D, also known as GOV, is a member of the Ras superfamily of small Rab GTPases. Rab proteins play an important role for, either in endocytosis or in biosynthetic protein transport. Rab3D mRNA is predominantly expressed in white adipose tissue and, coincident with GLuT4, increases upon differentiation of 3T3-L1 broblasts into insulin-responsive adipocytes. Rab3D displays close structural similarities with Rab 3A, an isoform which has been functionally implicated in regulated exocytosis in various cell types. Recombinant human Rab3D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RAB3D member RAS oncogene family, Ras-related protein Rab-3D, Rab3D upregulated with myeloid differentiation, glioblastoma overexpressed GOV, RAB16, D2-2, RAD3D

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.4 kDa (239aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RAB3D, Ras-related protein Rab-3D, GOV, RAB16, ATGP1102-10 µg, ATGP1102-20 µg, ATGP1102-50 µg, ATGP1102-100 µg, ATGP1102-250 µg, ATGP1102-500 µg, ATGP1102-1 mg, ATGP1102-10, ATGP1102-20, ATGP1102-50, ATGP1102-100, ATGP1102-250, ATGP1102-500, ATGP1102-1

Zahraoui A., et al. (1989) J Biol Chem. 264:12934-12401.; ; Baldini G., et al. (1992) Proc Natl Acad Sci. 89:5049-5052.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.2M NaCl

Signal Transduction

NP_004274

O95716

Human

MASAGDTQAG PRDAADQNFD YMFKLLLIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTVYRHDKR IKLQIWDTAG QERYRTITTA YYRGAMGFLL MYDIANQESF AAVQDWATQI KTYSWDNAQV ILVGNKCDLE DERVVPAEDG RRLADDLGFE FFEASAKENI NVKQVFERLV DVICEKMNES LEPSSSSGSN GKGPAVGDAP APQPSSCSC