Recombinant humaan RAB5B-eiwit

Ras-related protein Rab-5B, also known as RAB5B, is a member of the Rab family of small (monomeric) G proteins. RAB5B is involved in endocytosis and recycling of cell surface molecules. It interacts with RIN2 and RIN3, which regulate its function, possibly by acting as GEFs. Knockdown of RAB5B abolished group I metabotropic glutamate receptor (mGluR) -mediated neuroprotection. Recombinant human RAB5B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Rab-5B, Rab 5b, Rab5 b, RAB5B member RAS oncogene family, Ras related protein Rab 5B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.1 kDa (238aa) confirmed by MALDI-TOF

RAB5B, Ras-related protein Rab-5B, Rab 5b, Rab5 b, RAB5B member RAS oncogene family, Ras related protein Rab 5B, ATGP0846-10 µg, ATGP0846-20 µg, ATGP0846-50 µg, ATGP0846-100 µg, ATGP0846-250 µg, ATGP0846-500 µg, ATGP0846-1 mg, ATGP0846-10, ATGP0846-20, ATGP0846-50, ATGP0846-100, ATGP0846-250, ATGP0846-500, ATGP0846-1

Chavrier P., et al. (1992) Gene. 112:261-264.; ; Novick P., et al. (1993) Cell. 75:597-601.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.2M NaCl

Signal Transduction

NP_002859

P61020

Human

MTSRSTA RPNGQPQASK ICQFKLVLLG ESAVGKSSLV LRFVKGQFHE YQESTIGAAF LTQSVCLDDT TVKFEIWDTA GQERYHSLAP MYYRGAQAAI VVYDITNQET FARAKTWVKE LQRQASPSIV IALAGNKADL ANKRMVEYEE AQAYADDNSL LFMETSAKTA MNVNDLFLAI AKKLPKSEPQ NLGGAAGRSR GVDLHEQSQQ NKSQCCSN