Recombinant humaan RABL5/IFT22-eiwit

Rab-like protein 5 isoform a, also known as RABL5, is a member of the Ras superfamily of small GTP-binding proteins. The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies. Increasing data suggests an important role for Rab proteins in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Recombinant human RABL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Intraflagellar transport 22, Intraflagellar transport protein 22 homolog, Rab-like protein 5, RAB member RAS oncogene family-like 5, FAP9, CFAP9

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.2 kDa (208a) confirmed by MALDI-TOF

Rab-like protein 5 isoform a, Rab-like protein 5, RABL5, RAB member RAS oncogene family-like 5, RAB, Member RAS oncogene family-like 5, Intraflagellar transport protein 22 homolog, Intraflagellar transport 22, IFT22, FAP9, CFAP9, ATGP2109-10 µg, ATGP2109-20 µg, ATGP2109-50 µg, ATGP2109-100 µg, ATGP2109-250 µg, ATGP2109-500 µg, ATGP2109-1 mg, ATGP2109-010, ATGP2109-020, ATGP2109-050, ATGP2109-100, ATGP2109-250, ATGP2109-500, ATGP2109-01M

Adhiambo C., et al. (2009) J Cell Sci. 122:834-41

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_073614.1

Q9H7X7

Human

MLKAKIL FVGPCESGKT VLANFLTESS DITEYSPTQG VRILEFENPH VTSNNKGTGC EFELWDCGGD AKFESCWPAL MKDAHGVVIV FNADIPSHRK EMEMWYSCFV QQPSLQDTQC MLIAHHKPGS GDDKGSLSLS PPLNKLKLVH SNLEDDPEEI RMEFIKYLKS IINSMSESRD REEMSIMT