Recombinant humaan RAC1-eiwit

Rac1, belonging to the Rho small GTPase family, regulates the actin cytoskeleton but also other cellular processes. RAC1 have been shown to be involved in the regulation of cell-cell adhesion. Recombinant human RAC1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related C3 botulinum toxin substrate 1 isoform, MIG5, p21-Rac1, TC-25, Ras-related C3 botulinum toxin substrate 1 isoform Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, Rac 1, Ras like protein TC25, TC25.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

23.6 kDa (212aa) confirmed by MALDI-TOF

RAC1, Ras-related C3 botulinum toxin substrate 1 isoform, MIG5, p21-Rac1, TC-25, Ras-related C3 botulinum toxin substrate 1 isoform Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, Rac 1, Ras like protein TC25, TC25., ATGP0403-10 µg, ATGP0403-20 µg, ATGP0403-50 µg, ATGP0403-100 µg, ATGP0403-250 µg, ATGP0403-500 µg, ATGP0403-1 mg, ATGP0403-10, ATGP0403-20, ATGP0403-50, ATGP0403-100, ATGP0403-250, ATGP0403-500, ATGP0403-1

Chrostek A., et al. (2006) Mol Cell Biol. 26: 6957-70.; ; Sun CX., et al. (2004) Blood. 104 (12) :3758-65.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1mM EDTA, 10% glycerol

Cytoskeleton

NP_008839

P63000

Human

MGSSHHHHHH SSGLVPRGSH MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKCL LL