Recombinant humaan RACK1-eiwit

GNB2L1 was originally identified as an anchoring protein for protein kinase C beta (PKCbeta), which it stabilises in the active state and anchors to membranes or functional sites. However, evidence has accumulated to support a central role of GNB2L1 in critical biological responses. In addition to binding specifically to the active form of PKCbeta isoforms, GNB2L1 also interacts with several other important signaling proteins including the Src kinase family, integrin beta1, integrin beta2, integrin beta3 and integrin beta5, beta-spectrin and dynamin, RasGAP, the androgen receptor, insulin-like growth factor 1 receptor (IGF-1r), Epstein-Barr virus trans-activator protein BZLF1, p73 and pRB. GNB2L1 is ubiquitously expressed in the tissues of higher mammals and humans including brain, liver, and spleen. Recombinant human GNB2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-2-like 1, Gnb2-rs1, H12.3, HLC-7, PIG21, RACK1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.2 kDa (337aa) confirmed by MALDI-TOF

RACK1, Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-2-like 1, Gnb2-rs1, H12.3, HLC-7, PIG21, RACK1, ATGP1375-10 µg, ATGP1375-20 µg, ATGP1375-50 µg, ATGP1375-100 µg, ATGP1375-250 µg, ATGP1375-500 µg, ATGP1375-1 mg, ATGP1375-10, ATGP1375-20, ATGP1375-50, ATGP1375-100, ATGP1375-250, ATGP1375-500, ATGP1375-1

Chang B.Y., et al. (1998) Mol. Cell. Biol. 18:3245-3256; ; Chang B.Y., et al. (2001) J. Biol. Chem. 276:20346-20356

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT

Signal Transduction

NP_006089.1

P63244

Human

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA GYTDNLVRVW QVTIGTR