Recombinant humaan RAD1-eiwit

RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

33.9 kDa (302aa) confirmed by MALDI-TOF

RAD1, Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1, ATGP0645-10 µg, ATGP0645-20 µg, ATGP0645-50 µg, ATGP0645-100 µg, ATGP0645-250 µg, ATGP0645-500 µg, ATGP0645-1 mg, ATGP0645-10, ATGP0645-20, ATGP0645-50, ATGP0645-100, ATGP0645-250, ATGP0645-500, ATGP0645-1

Liu Y., et al. (2010) J Biol Chem. 285 (8) :5974-82.; ; Yazinski SA., et al. (2009) Proc Natl Acad Sci u S A. 106 (50) :21282-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol

Cell Cycle

NP_002844.1

O60671

Human

MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES