Recombinant humaan RalB-eiwit

RALB, also called Ras-related protein Ral-B, belongs to subfamily of Ras-related GTPases. This protein is activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. It plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Also it is required for suppression of apoptosis. Recombinant human RALB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ras-related protein Ral-B, RAS like proto-oncogene B, Ras related GTP binding protein B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.6 kDa (227aa) confirmed by MALDI-TOF

RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, ATGP0839-10 µg, ATGP0839-20 µg, ATGP0839-50 µg, ATGP0839-100 µg, ATGP0839-250 µg, ATGP0839-500 µg, ATGP0839-1 mg, ATGP0839-010, ATGP0839-020, ATGP0839-050, ATGP0839-100, ATGP0839-250, ATGP0839-500, ATGP0839-01M

Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.; ; Jullien-Flores V., et al. (1995) . J. Biol. Chem. 270: 22473-22477

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_002872

P11234

Human

HMAANKS KGQSSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLLVFSIT EHESFTATAE FREQILRVKA EEDKIPLLVV GNKSDLEERR QVPVEEARSK AEEWGVQYVE TSAKTRANVD KVFFDLMREI RTKKMSENKD KNGKKSSKNK KSFKERC