Recombinant humaan RAMP1-eiwit

RAMP is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs) . RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Recombinant human RAMP1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Receptor activity-modifying protein 1 precursor, CRLR activity-modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, Receptor activity-modifying protein 1, receptor (G protein-coupled) activity modifying protein 1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

12.9 kDa (114aa)

RAMP1, Receptor activity-modifying protein 1 precursor, CRLR activity-modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, Receptor activity-modifying protein 1, receptor (G protein-coupled) activity modifying protein 1, ATGP2518-10 µg, ATGP2518-20 µg, ATGP2518-50 µg, ATGP2518-100 µg, ATGP2518-250 µg, ATGP2518-500 µg, ATGP2518-1 mg, ATGP2518-10, ATGP2518-20, ATGP2518-50, ATGP2518-100, ATGP2518-250, ATGP2518-500, ATGP2518-1

Kusano S, Kukimoto-Niino M, et al. (2008) . Protein Sci. 17 (11) :1907-14. ; ; Heroux M, Hogue M, et al. (2007) . J Biol Chem. 282 (43) :31610-20.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Signal Transduction

NP_005846

O60894

Human

MGSSHHHHHH SSGLVPRGSH MGSCQEANYG ALLRELCLTQ FQVDMEAVGE TLWCDWGRTI RSYRELADCT WHMAEKLGCF WPNAEVDRFF LAVHGRYFRS CPISGRAVRD PPGS