Recombinant humaan RAMP3-eiwit

RAMP3, also known as receptor activity modifying protein 3, is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs) . It is required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor. Recombinant human RAMP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Receptor activity modifying protein 3, Receptor (calcitonin) activity modifying protein 3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

13.0 kDa (116aa) confirmed by MALDI-TOF

RAMP3, Receptor activity modifying protein 3, Receptor (calcitonin) activity modifying protein 3, ATGP1970-10 µg, ATGP1970-20 µg, ATGP1970-50 µg, ATGP1970-100 µg, ATGP1970-250 µg, ATGP1970-500 µg, ATGP1970-1 mg, ATGP1970-10, ATGP1970-20, ATGP1970-50, ATGP1970-100, ATGP1970-250, ATGP1970-500, ATGP1970-1

Kuwasako K. et al. (2008) Biochem Biophys Res Commun. 377:109-113.; ; Roh J. et al. (2004) J Biol Chem. 279:7264-7274;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Signal Transduction

NP_005847

O60896

Human

RAGGCNETG MLERLPLCGK AFADMMGKVD VWKWCNLSEF IVYYESFTNC TEMEANVVGC YWPNPLAQGF ITGIHRQFFS NCTVDRVHLE DPPDEV