Recombinant humaan RAN-eiwit

RAN, member RAS oncogene family, also known as RAN, is a small GTPase of the Ras superfamily that controls nucleocytoplasmic transport. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. unlike many other regulatory GTPases, Ran is not posttranslationally modified so that it can be targeted to the subcellular compartment where it acts. Rather, RanGTP has been thought to be localized by the differential localization of Ran regulators. Recombinant human RAN, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

RAN member RAS oncogene family, ARA24, Gsp1, TC4, RAN, member RAS oncogene family, Androgen receptor associated protein 24, ARA 24, GTP binding nuclear protein RAN, GTPase Ran, LPS, RAN member RAS oncogene family, Ras like protein TC4, RASL2 8

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.5 kDa (236aa) confirmed by MALDI-TOF

RAN, RAN member RAS oncogene family, ARA24, Gsp1, TC4, RAN, member RAS oncogene family, Androgen receptor associated protein 24, ARA 24, GTP binding nuclear protein RAN, GTPase Ran, LPS, RAN member RAS oncogene family, Ras like protein TC4, RASL2 8, ATGP0590-10 µg, ATGP0590-20 µg, ATGP0590-50 µg, ATGP0590-100 µg, ATGP0590-250 µg, ATGP0590-500 µg, ATGP0590-1 mg, ATGP0590-10, ATGP0590-20, ATGP0590-50, ATGP0590-100, ATGP0590-250, ATGP0590-500, ATGP0590-1

Woo IS., et al. (2008) Apoptosis. 13 (10) :1223-31.; ; Ren M., et al. (1994) Mol Cell biol. 14 (6) :4216-24.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Signal Transduction

NP_006316

P62826

Human

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL