Recombinant humaan RANBP1-eiwit

RAN binding protein 1, also known as RANBP1, belongs to a family of proteins that bind Ran GTPase which is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1) . Also, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. Recombinant human RANBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RAN binding protein 1, HTF9A

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.8 kDa (225aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RANBP1, RAN binding protein 1, HTF9A, ATGP1466-10 µg, ATGP1466-20 µg, ATGP1466-50 µg, ATGP1466-100 µg, ATGP1466-250 µg, ATGP1466-500 µg, ATGP1466-1 mg, ATGP1466-10, ATGP1466-20, ATGP1466-50, ATGP1466-100, ATGP1466-250, ATGP1466-500, ATGP1466-1

Hayashi N., et al. (1995) Mol Gen Genet. 247 (6) :661-9.; ; Ren M., et al. (1995) Mol Cell biol. 15 (4) :2177-24.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Epigenomics (Transcription & Translation)

NP_002873

P43487

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ