Recombinant humaan RANGRF-eiwit

RANGRF is a protein that has been shown to function as a guanine nucleotide release factor in mouse and to regulate the expression and function of the Nav1. 5 cardiac sodium channel in human. Alternative splicing results in multiple transcript variants. Recombinant human RANGRF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RAN guanine nucleotide release factor, HSPC165, HSPC236, MOG1, RANGNRF

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23 kDa (210aa) confirmed by MALDI-TOF

RANGRF, RAN guanine nucleotide release factor, HSPC165, HSPC236, MOG1, RANGNRF, ATGP1620-10 µg, ATGP1620-20 µg, ATGP1620-50 µg, ATGP1620-100 µg, ATGP1620-250 µg, ATGP1620-500 µg, ATGP1620-1 mg, ATGP1620-10, ATGP1620-20, ATGP1620-50, ATGP1620-100, ATGP1620-250, ATGP1620-500, ATGP1620-1

Olesen, M.S., et al. (2011) Can J Cardiol 27 (4), 523; ; Kattygnarath, D., et al. (2011) Circ Cardiovasc Genet 4 (3), 261-268.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl, 1mM DTT

Signal Transduction

NP_057576

Q9HD47

Human

MEPTRD CPLFGGAFSA ILPMGAIDVS DLRPVPDNQE VFCHPVTDQS LIVELLELQA HVRGEAAARY HFEDVGGVQG ARAVHVESVQ PLSLENLALR GRCQEAWVLS GKQQIAKENQ QVAKDVTLHQ ALLRLPQYQT DLLLTFNQPP PDNRSSLGPE NLSPAPWSLG DFEQLVTSLT LHDPNIFGPQ