Recombinant humaan Rap1A-eiwit

Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ras-related protein Rap-1A, KREV1, G-22K, C21KG, Ras-related protein Rap-1A, G 22K, GTP binding protein smg p21A, KREV 1, OTTHuMP00000013741, RAP 1A, RAP1, RAP1A member of RAS oncogene family, Ras related protein Krev 1, Ras related protein Rap 1A, RAS related protein RAP1A, SMGP21

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

22.8 kDa (201aa) confirmed by MALDI-TOF

Rap1A, Ras-related protein Rap-1A, KREV1, G-22K, C21KG, Ras-related protein Rap-1A, G 22K, GTP binding protein smg p21A, KREV 1, OTTHuMP00000013741, RAP 1A, RAP1, RAP1A member of RAS oncogene family, Ras related protein Krev 1, Ras related protein Rap 1A, RAS related protein RAP1A, SMGP21, ATGP0598-10 µg, ATGP0598-20 µg, ATGP0598-50 µg, ATGP0598-100 µg, ATGP0598-250 µg, ATGP0598-500 µg, ATGP0598-1 mg, ATGP0598-10, ATGP0598-20, ATGP0598-50, ATGP0598-100, ATGP0598-250, ATGP0598-500, ATGP0598-1

Han, L., et al. (1995) Mol Cell Biol. 15 (3) : 1318-23.; ; Rebhun, J F, et al. (2000) J Biol Chem. 275 (45) : 34901-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl

Signal Transduction

NP_001010935

P62834

Human

MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C