Recombinant humaan RAP1B-eiwit

RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras-related protein Rap-1b, DKFZp586H0723, K-REV, RAL1B, RAP1B member of RAS oncogene family, GTP-binding protein smg p21B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.6 kDa (201aa) confirmed by MALDI-TOF

RAP1B, ATGP0891-10 µg, ATGP0891-20 µg, ATGP0891-50 µg, ATGP0891-100 µg, ATGP0891-250 µg, ATGP0891-500 µg, ATGP0891-1 mg, ATGP0891-10, ATGP0891-20, ATGP0891-50, ATGP0891-100, ATGP0891-250, ATGP0891-500, ATGP0891-1

Awasthi A., et al. (2010) J Exp Med. 207 (9) :1923-38.; ; Schoenwaelder SM., et al. (2010) J Biol Chem. 285 (4) :2886-96.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 5.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Signal Transduction

NP_056461

P61224

Human

MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C