Recombinant humaan RBBP4-eiwit

Retinoblastoma binding protein 4, also known as RBBP4, is a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. RBBP4 is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. Recombinant human RBBP4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Retinoblastoma binding protein 4, NuRF55, RBAP48

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50.2 kDa (449aa)

RBBP4, Retinoblastoma binding protein 4, NuRF55, RBAP48, ATGP1656-10 µg, ATGP1656-20 µg, ATGP1656-50 µg, ATGP1656-100 µg, ATGP1656-250 µg, ATGP1656-500 µg, ATGP1656-1 mg, ATGP1656-10, ATGP1656-20, ATGP1656-50, ATGP1656-100, ATGP1656-250, ATGP1656-500, ATGP1656-1

Qian YW., et al. (1993) Nature. 364 (6438) : 648-52; ; Nicolas E., et al. (2001) Nucleic Acids Res. 29 (15) : 3131-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA

Epigenomics (Transcription & Translation)

NP_005601

Q09028

Human

MGSSHHHHHH SSGLVPRGSH MGSHMADKEA AFDDAVEERV INEEYKIWKK NTPFLYDLVM THALEWPSLT AQWLPDVTRP EGKDFSIHRL VLGTHTSDEQ NHLVIASVQL PNDDAQFDAS HYDSEKGEFG GFGSVSGKIE IEIKINHEGE VNRARYMPQN PCIIATKTPS SDVLVFDYTK HPSKPDPSGE CNPDLRLRGH QKEGYGLSWN PNLSGHLLSA SDDHTICLWD ISAVPKEGKV VDAKTIFTGH TAVVEDVSWH LLHESLFGSV ADDQKLMIWD TRSNNTSKPS HSVDAHTAEV NCLSFNPYSE FILATGSADK TVALWDLRNL KLKLHSFESH KDEIFQVQWS PHNETILASS GTDRRLNVWD LSKIGEEQSP EDAEDGPPEL LFIHGGHTAK ISDFSWNPNE PWVICSVSED NIMQVWQMAE NIYNDEDPEG SVDPEGQGS