Recombinant humaan RBM17-eiwit

RNA binding motif protein 17, also known as RBM17, is splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. This protein is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. This protein promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. Recombinant human RBM17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RNA binding motif protein 17, SPF45

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.1 kDa (421aa) confirmed by MALDI-TOF

RBM17, RNA binding motif protein 17, SPF45, ATGP2451-10 µg, ATGP2451-20 µg, ATGP2451-50 µg, ATGP2451-100 µg, ATGP2451-250 µg, ATGP2451-500 µg, ATGP2451-1 mg, ATGP2451-10, ATGP2451-20, ATGP2451-50, ATGP2451-100, ATGP2451-250, ATGP2451-500, ATGP2451-1

Lallena M.J., et al. (2002) Cell. 109:285-296; ; Carrascal M., et al. (2008) J. Proteome Res. 7:5167-5176

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_116294.1

Q96I25

Human

MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V