Recombinant humaan RBM3-eiwit

RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recombinant human RBM3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Putative RNA-binding protein 3, IS1-RNPL; RNPL

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19 kDa (180aa) confirmed by MALDI-TOF

RBM3, Putative RNA-binding protein 3, IS1-RNPL; RNPL, ATGP2097-10 µg, ATGP2097-20 µg, ATGP2097-50 µg, ATGP2097-100 µg, ATGP2097-250 µg, ATGP2097-500 µg, ATGP2097-1 mg, ATGP2097-10, ATGP2097-20, ATGP2097-50, ATGP2097-100, ATGP2097-250, ATGP2097-500, ATGP2097-1

Dellis S, Strickland KC, et al. (2004) . Virology. 329 (2) :328-36.; ; Martinez-Arribas F, Agudo D, et al. (2006) . J Cell Biochem. 97 (6) :1275-82.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT

Metabolism

NP_006734

P98179

Human

MSSEEGK LFVGGLNFNT DEQALEDHFS SFGPISEVVV VKDRETQRSR GFGFITFTNP EHASVAMRAM NGESLDGRQI RVDHAGKSAR GTRGGGFGAH GRGRSYSRGG GDQGYGSGRY YDSRPGGYGY GYGRSRDYNG RNQGGYDRYS GGNYRDNYDN