Recombinant humaan RBM8A-eiwit

RBM8A is a member of the EJC (The exon junction complex) that is involved in mRNA export, cytoplasmic localization and nonsense mediated mRNA decay. This protein has the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. Although it shuttles to the cytoplasm, it is predominantly detected in the nucleus and is colocalized with oskar mRNA at the posterior pole of the cell. Recombinant human RBM8A protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

RNA-binding protein 8A, BOV-1A, BOV-1B, BOV-1C, MDS014, RBM8, RBM8B, Y14, ZNRP, ZRNP1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

20.9 kDa (182aa) confirmed by MALDI-TOF

RBM8A, RNA-binding protein 8A, BOV-1A, BOV-1B, BOV-1C, MDS014, RBM8, RBM8B, Y14, ZNRP, ZRNP1, ATGP0990-10 µg, ATGP0990-20 µg, ATGP0990-50 µg, ATGP0990-100 µg, ATGP0990-250 µg, ATGP0990-500 µg, ATGP0990-1 mg, ATGP0990-10, ATGP0990-20, ATGP0990-50, ATGP0990-100, ATGP0990-250, ATGP0990-500, ATGP0990-1

Reichert V.L. et al. (2002) Genes Dev., 16: 2778-2791.; ; Dostie J. et al. (2002) Curr. Biol. 12: 1060-1067.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Epigenomics (Transcription & Translation)

NP_005096.1

Q9Y5S9

Human