Recombinant humaan RCAN2-eiwit

Calcipressin-2, also known as RCAN2, belonging to the RCAN (Regulator of calcineurin) family modulate the activity of Calcineurin through a valine-rich region within their carboxyl tail. RCANs compete with NFAT (nuclear factor of activated T-cells) for binding to the same docking site in calcineurin, which results in either inhibition or activation of calcineurin activity. RCAN2 is a Ca (2+) dependent protein phosphatase that is involed in several neuornal functions. Recombinant human RCAN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Calcipressin-2, CSP2, DSCR1L1, hRCN2, MCIP2, RCN2, ZAKI-4, ZAKI4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.1 kDa (217aa) confirmed by MALDI-TOF

RCAN2, Calcipressin-2, CSP2, DSCR1L1, hRCN2, MCIP2, RCN2, ZAKI-4, ZAKI4, ATGP1309-10 µg, ATGP1309-20 µg, ATGP1309-50 µg, ATGP1309-100 µg, ATGP1309-250 µg, ATGP1309-500 µg, ATGP1309-1 mg, ATGP1309-10, ATGP1309-20, ATGP1309-50, ATGP1309-100, ATGP1309-250, ATGP1309-500, ATGP1309-1

Qin L., et al. (2006) Mal Cancer Res. 4:811-820.; ; Porta S., et al. (2007) Eur J Neurosci. 26:1213-1226.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.2M NaCl

Signal Transduction

NP_005813

Q14206

Human

MGSSHHHHHH SSGLVPRGSH MPAPSMDCDV STLVACVVDV EVFTNQEVKE KFEGLFRTYD DCVTFQLFKS FRRVRINFSN PKSAARARIE LHETQFRGKK LKLYFAQVQT PETDGDKLHL APPQPAKQFL ISPPSSPPVG WQPINDATPV LNYDLLYAVA KLGPGEKYEL HAGTESTPSV VVHVCDSDIE EEEDPKTSPK PKIIQTRRPG LPPSVSN