Recombinant humaan RCAN3-eiwit

Calcipressin-3, also known as RCAN3, is a 241 amino acid protein that potentially is involved in central nervous system development. RCAN3 inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Overexpression of calcipressin-3 results in inhibition of calcineurin activity towards the nuclear factor of activated T-cells (NFAT) transcription factors and also downregulates NFAT-dependent cytokine gene expression in activated Jurkat T-cells. Highest expression occurs in heart, skeletal muscle kidney, liver and peripheral blood leukocytes. Recombinant human RCAN3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Calcipressin-3, DSCR1L2, hRCN3, MCIP3, RCN3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.5 kDa (209aa)

RCAN3, Calcipressin-3, DSCR1L2, hRCN3, MCIP3, RCN3, ATGP1811-10 µg, ATGP1811-20 µg, ATGP1811-50 µg, ATGP1811-100 µg, ATGP1811-250 µg, ATGP1811-500 µg, ATGP1811-1 mg, ATGP1811-10, ATGP1811-20, ATGP1811-50, ATGP1811-100, ATGP1811-250, ATGP1811-500, ATGP1811-1

Strippoli P., et al. (2000) Genomics. 64:252-263.; ; Abbasi S., et al. (2006) J Biol Chem. 281:7717-7726.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol, 0.2M NaCl

Neuroscience

NP_038469

Q9UKA8

Human

MGSSHHHHHH SSGLVPRGSH MGSEAVFEAR EQKERFEALF TIYDDQVTFQ LFKSFRRVRI NFSKPEAAAR ARIELHETDF NGQKLKLYFA QVQMSGEVRD KSYLLPPQPV KQFLISPPAS PPVGWKQSED AMPVINYDLL CAVSKLGPGE KYELHAGTES TPSVVVHVCE SETEEEEETK NPKQKIAQTR RPDPPTAALN EPQTFDCAL