Recombinant humaan RCHY1-eiwit

RCHY1 has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Recombinant human RCHY1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

RING finger and CHY zinc finger domain-containing protein 1, ARNIP, CHIMP, hARNIP, PIRH2, PIRH2E, PIRH2F, PRO1996, RNF199, ZNF363

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.5 kDa (284aa)

RCHY1, RING finger and CHY zinc finger domain-containing protein 1, ARNIP, CHIMP, hARNIP, PIRH2, PIRH2E, PIRH2F, PRO1996, RNF199, ZNF363, ATGP2187-10 µg, ATGP2187-20 µg, ATGP2187-50 µg, ATGP2187-100 µg, ATGP2187-250 µg, ATGP2187-500 µg, ATGP2187-1 mg, ATGP2187-10, ATGP2187-20, ATGP2187-50, ATGP2187-100, ATGP2187-250, ATGP2187-500, ATGP2187-1

Hu, L., et al. (2012) Carcinogenesis 33 (8), 1581-1588; ; Wang, J., et al. (2012) Pharmacogenomics J.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Cancer

NP_056251

Q96PM5

Human

MAATARE DGASGQERGQ RGCEHYDRGC LLKAPCCDKL YTCRLCHDNN EDHQLDRFKV KEVQCINCEK IQHAQQTCEE CSTLFGEYYC DICHLFDKDK KQYHCENCGI CRIGPKEDFF HCLKCNLCLA MNLQGRHKCI ENVSRQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HSALDMTRYW RQLDDEVAQT PMPSEYQNMT VDILCNDCNG RSTVQFHILG MKCKICESYN TAQAGGRRIS LDQQ