Recombinant humaan RCN3-eiwit

Reticulocalbin 3, also known as RCN3, is a member of the CREC (cab45/reticulocalbin/ ERC45/calumenin) family. RCN3 contains five Arg-Xaa-Xaa-Arg motifs, which function as target sequences of SPCs (subtilisin-like proprotein convertases), a family of serine endoproteases that proteolytically activate proproteins. The synthesis of one such member, PACE4 (paired basic amino acid cleaving enzyme 4), is influenced by association and coexpression with reticulocalbin-3. Recombinant human RCN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Reticulocalbin 3, RLP49

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.9 kDa (333aa) confirmed by MALDI-TOF

RCN3, Reticulocalbin 3, RLP49, ATGP1434-10 µg, ATGP1434-20 µg, ATGP1434-50 µg, ATGP1434-100 µg, ATGP1434-250 µg, ATGP1434-500 µg, ATGP1434-1 mg, ATGP1434-10, ATGP1434-20, ATGP1434-50, ATGP1434-100, ATGP1434-250, ATGP1434-500, ATGP1434-1

Teglund S., et al. (1994) Genomics. 23:669-684.; ; Tsuji A., et al. (2006) Biochem J. 396:51-59.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Signal Transduction

NP_065701

Q96D15

Human

MGSSHHHHHH SSGLVPRGSH MGSHMKPSPD AGPHGQGRVH QAAPLSDAPH DDAHGNFQYD HEAFLGREVA KEFDQLTPEE SQARLGRIVD RMDRAGDGDG WVSLAELRAW IAHTQQRHIR DSVSAAWDTY DTDRDGRVGW EELRNATYGH YAPGEEFHDV EDAETYKKML ARDERRFRVA DQDGDSMATR EELTAFLHPE EFPHMRDIVI AETLEDLDRN KDGYVQVEEY IADLYSAEPG EEEPAWVQTE RQQFRDFRDL NKDGHLDGSE VGHWVLPPAQ DQPLVEANHL LHESDTDKDG RLSKAEILGN WNMFVGSQAT NYGEDLTRHH DEL