Recombinant humaan RDBP/NELFE-eiwit

RDBP, also known as NELF-E, is a putative RNA binding protein. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions. Recombinant human RDBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Negative elongation factor complex member E, RD RNA-binding protein, RNA-binding protein RD, RD, D6S45, NELF-E, RDP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

45.4 kDa (400aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RDBP, RD RNA binding protein, D6S45, NELF-E, RD, RDP, NELFe, ATGP0974-10 µg, ATGP0974-20 µg, ATGP0974-50 µg, ATGP0974-100 µg, ATGP0974-250 µg, ATGP0974-500 µg, ATGP0974-1 mg, ATGP0974-10, ATGP0974-20, ATGP0974-50, ATGP0974-100, ATGP0974-250, ATGP0974-500, ATGP0974-1

Wada T., et al. (2000) Mol Cell. 5 (6) :1067-72.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1mM PMSF

Epigenomics (Transcription & Translation)

NP_002895

P18615

Human

MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF