Recombinant humaan RDBP/NELFE-eiwit

NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D) . Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Negative elongation factor complex member E, RD RNA-binding protein, RNA-binding protein RD, RD, D6S45, NELF-E, RDP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BRADFORD assay)

> 90% by SDS-PAGE

45.6 kDa (403aa) confirmed by MALDI-TOF

NELFE, Negative elongation factor E, Negative elongation factor complex member E, D6S45, NELF-E, RD, RDBP, RDP, ATGP2685-10 µg, ATGP2685-20 µg, ATGP2685-50 µg, ATGP2685-100 µg, ATGP2685-250 µg, ATGP2685-500 µg, ATGP2685-1 mg, ATGP2685-10, ATGP2685-20, ATGP2685-50, ATGP2685-100, ATGP2685-250, ATGP2685-500, ATGP2685-1

Yamaguchi Y., Takagi T, et al. (1999) Cell 97:41-51; ; Surowy C.S., Hoganson G, et al. (1990) Gene 90:299-302;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT

Epigenomics (Transcription & Translation)

NP_002895

P18615

Human

MLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF