Recombinant humaan RDH12-eiwit

Retinol dehydrogenase 12, also known as RDH12, belongs to the short-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed, mostly in eye, kidney, brain, skeletal muscle and stomach. It is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Recombinant human RDH12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Retinol dehydrogenase 12, LCA13, LCA3, SDR7C2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.5 kDa (303aa) confirmed by MALDI-TOF

RDH12, Retinol dehydrogenase 12, LCA13, LCA3, SDR7C2, ATGP1371-10 µg, ATGP1371-20 µg, ATGP1371-50 µg, ATGP1371-100 µg, ATGP1371-250 µg, ATGP1371-500 µg, ATGP1371-1 mg, ATGP1371-10, ATGP1371-20, ATGP1371-50, ATGP1371-100, ATGP1371-250, ATGP1371-500, ATGP1371-1

Maeda A., et al. (2006) J Biol Chem. 281:37697-37704.; ; Keller B., et al. (2007) J Steroid Biochem Mol Biol. 104:190-194.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 0.2M NaCl

Metabolism

NP_689656.2

Q96NR8

Human

GKVVV ITGANTGIGK ETARELASRG ARVYIACRDV LKGESAASEI RVDTKNSQVL VRKLDLSDTK SIRAFAEGFL AEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKI PFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSP FVKTAREGAQ TSLHCALAEG LEPLSGKYFS DCKRTWVSPR ARNNKTAERL WNVSCELLGI RWE