Recombinant humaan Retinol Bindeiwit 7/RBP7 eiwit

RBP7, also known as CRBP4, belongs to a superfamily of small cytoplasmic proteins which interact with hydrophobic ligands. RBP7 is cytoplasmic protein that, like CRBP I and CRBP II, form beta-barrel structures and participates in the intracellular transport of retinol. RBP7 is a recently identified cellular retinol carrier, expressed in the kidney, heart and transverse colon in humans. Recombinant human RBP7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Retinoid-binding protein 7, CRBP4, CRBPIV, MGC70641, Cellular retinoic acid-binding protein 4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.6 kDa (154aa) confirmed by MALDI-TOF

RBP7, Retinoid-binding protein 7, CRBP4, CRBPIV, MGC70641, ATGP0936-10 µg, ATGP0936-20 µg, ATGP0936-50 µg, ATGP0936-100 µg, ATGP0936-250 µg, ATGP0936-500 µg, ATGP0936-1 mg, ATGP0936-10, ATGP0936-20, ATGP0936-50, ATGP0936-100, ATGP0936-250, ATGP0936-500, ATGP0936-1

Aoki T., et al. (2009) Clin Exp Allergy. 39 (2) :213-21.; ; Sampath V., et al. (2008) J Biol Chem. 283 (7) :3923-31.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 20% glycerol

Metabolism

NP_443192

Q96R05

Human

MGSSHHHHHH SSGLVPRGSH MPADLSGTWT LLSSDNFEGY MLALGIDFAT RKIAKLLKPQ KVIEQNGDSF TIHTNSSLRN YFVKFKVGEE FDEDNRGLDN RKCKSLVIWD NDRLTCIQKG EKKNRGWTHW IEGDKLHLEM FCEGQVCKQT FQRA