Recombinant humaan REV1-eiwit

REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

DNA repair protein REV1, DNA repair protein REV1, REV1L

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.2 kDa (227aa)

REV1, DNA repair protein REV1, DNA repair protein REV1, REV1L, ATGP2668-10 µg, ATGP2668-20 µg, ATGP2668-50 µg, ATGP2668-100 µg, ATGP2668-250 µg, ATGP2668-500 µg, ATGP2668-1 mg, ATGP2668-10, ATGP2668-20, ATGP2668-50, ATGP2668-100, ATGP2668-250, ATGP2668-500, ATGP2668-1

Gibbs P.E.M., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:4186-4191; ; Masuda Y., et al. (2001) J. Biol. Chem. 276:15051-15058;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_057400

Q9UBZ9

Human

MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA