Recombinant humaan RFXANK-eiwit

RFXANK, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. Recombinant human RFXANK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

DNA-binding protein RFXANK isoform b, ANKRA1, BLS, F14150_1, RFX-B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28 kDa (260aa) confirmed by MALDI-TOF

RFXANK, DNA-binding protein RFXANK isoform b, ANKRA1, BLS, F14150_1, RFX-B, ATGP2188-10 µg, ATGP2188-20 µg, ATGP2188-50 µg, ATGP2188-100 µg, ATGP2188-250 µg, ATGP2188-500 µg, ATGP2188-1 mg, ATGP2188-10, ATGP2188-20, ATGP2188-50, ATGP2188-100, ATGP2188-250, ATGP2188-500, ATGP2188-1

Krawczyk M, Masternak K, et al. (2005) . Mol Cell Biol. 25 (19) :8607-18.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Immunology

NP_604389

O14593

Human

MELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE