Recombinant humaan RGS1-eiwit

RGS1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Recombinant human RGS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Regulator of G-protein signaling 1, 1R20; BL34; IER1; IR20

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.4 kDa (233aa) confirmed by MALDI-TOF

RGS1, Regulator of G-protein signaling 1, 1R20; BL34; IER1; IR20, ATGP1572-10 µg, ATGP1572-20 µg, ATGP1572-50 µg, ATGP1572-100 µg, ATGP1572-250 µg, ATGP1572-500 µg, ATGP1572-1 mg, ATGP1572-10, ATGP1572-20, ATGP1572-50, ATGP1572-100, ATGP1572-250, ATGP1572-500, ATGP1572-1

Bowman EP., et al. (1998) . J. Biol. Chem. 273 (43) : 28040-8.; ; Hoffmann M., et al. (2001) . J. Neurochem. 78 (4) : 797-806

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_002913

Q08116

Human

MRAAAI STPKLDKMPG MFFSANPKEL KGTTHSLLDD KMQKRRPKTF GMDMKAYLRS MIPHLESGMK SSKSKDVLSA AEVMQWSQSL EKLLANQTGQ NVFGSFLKSE FSEENIEFWL ACEDYKKTES DLLPCKAEEI YKAFVHSDAA KQINIDFRTR ESTAKKIKAP TPTCFDEAQK VIYTLMEKDS YPRFLKSDIY LNLLNDLQAN SLK