Recombinant humaan RGS17-eiwit

RGS17, also known as regulator of G-protein signaling 17, attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. It plays an important role in termination of signalling by mu opioid receptors and development of tolerance to opioid analgesic drugs. Recombinant human RGS17 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Regulator of G-protein signaling 17, hRGS17, RGS-17, RGSZ2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.5 kDa (230aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

RGS17, Regulator of G-protein signaling 17, hRGS17, RGS-17, RGSZ2, ATGP1200-10 µg, ATGP1200-20 µg, ATGP1200-50 µg, ATGP1200-100 µg, ATGP1200-250 µg, ATGP1200-500 µg, ATGP1200-1 mg, ATGP1200-10, ATGP1200-20, ATGP1200-50, ATGP1200-100, ATGP1200-250, ATGP1200-500, ATGP1200-1

Sierra D.A. et al. (2002) Genomics 79: 177-185.; ; Mao H. et al. (2004) J. Biol. Chem. 279: 26314-26322.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_036551

Q9UGC6

Human

MGSSHHHHHH SSGLVPRGSH MRKRQQSQNE GTPAVSQAPG NQRPNNTCCF CWCCCCSCSC LTVRNEERGE NAGRPTHTTK MESIQVLEEC QNPTAEEVLS WSQNFDKMMK APAGRNLFRE FLRTEYSEEN LLFWLACEDL KKEQNKKVIE EKARMIYEDY ISILSPKEVS LDSRVREVIN RNLLDPNPHM YEDAQLQIYT LMHRDSFPRF LNSQIYKSFV ESTAGSSSES