Recombinant humaan RhoA-eiwit

RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila) . Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ras homolog gene family member A, ARH12, ARHA, RHO12, RHOH12, Transforming protein RhoA, Rho cDNA clone 12

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.7 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Ras homolog gene family, member A, Ras homolog gene family member A, ARH12, ARHA, RHO12, RHOH12, Transforming protein RhoA, Rho cDNA clone 12, RHOA, ATGP0849-10 µg, ATGP0849-20 µg, ATGP0849-50 µg, ATGP0849-100 µg, ATGP0849-250 µg, ATGP0849-500 µg, ATGP0849-1 mg, ATGP0849-010, ATGP0849-020, ATGP0849-050, ATGP0849-100, ATGP0849-250, ATGP0849-500, ATGP0849-01M

Madaule P. et al. (1985) Cell. 41:31-40; ; Garrett M.D. et al. (1989) J. Biol. Chem. 264:10-13

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Cancer

NP_001655.1

P61586

Human

MAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC