Recombinant humaan RhoC-eiwit

RhoC, also known as rho-related GTP-binding protein RhoC, is a small signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. RhoC promotes reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Recombinant human RhoC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Rho-related GTP-binding protein RhoC, ARH9, ARHC, H9, RHOH9, Rho-related GTP-binding protein RhoC, Ras homolog family member C, Rho cDNA clone 9

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.8 (210aa) confirmed by MALDI-TOF (Real Molecular weight on SDS-PAGE will be shift up)

RhoC, Rho-related GTP-binding protein RhoC, ARH9, ARHC, H9, RHOH9, Rho-related GTP-binding protein RhoC, ATGP0638-10 µg, ATGP0638-20 µg, ATGP0638-50 µg, ATGP0638-100 µg, ATGP0638-250 µg, ATGP0638-500 µg, ATGP0638-1 mg, ATGP0638-10, ATGP0638-20, ATGP0638-50, ATGP0638-100, ATGP0638-250, ATGP0638-500, ATGP0638-1

Ridley AJ.. (2006) Trends Cell Biol. 16 (10) :522-9

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Signal Transduction

NP_001036144

P08134

Human

MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYIADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS AKTKEGVREV FEMATRAGLQ VRKNKRRRGC