Recombinant humaan Rhodanese/TST-eiwit

Thiosulfate sulfurtransferase (TST), also known as Rhodanese, is a mitochondrial enzyme that involved in cyanide detoxification and the modification of sulfur-containing enzymes. This protein contains two highly conservative domains, known as rhodanese homology domains. In mammals, most cyanide is converted to thiocyanate by this enzyme. TST also has weak mercaptopyruvate sulfurtransferase activity. Recombinant TST protein was expressed in E. coli and purified by using conventional chromatography techniques.

RDS, TST, Rhodanese, Thiosulfate Sulfurtransferase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.5 kDa (317aa) confirmed by MALDI-TOF

Thiosulfate sulfurtransferase, RDS, TST, Rhodanese, Thiosulfate Sulfurtransferase, ATGP0423-10 µg, ATGP0423-20 µg, ATGP0423-50 µg, ATGP0423-100 µg, ATGP0423-250 µg, ATGP0423-500 µg, ATGP0423-1 mg, ATGP0423-10, ATGP0423-20, ATGP0423-50, ATGP0423-100, ATGP0423-250, ATGP0423-500, ATGP0423-1

Pallini R., et al. (1991) Biochem Biophys Res Commun. 180 (2) :887-93. ; ; Aita N., et al. (1997) Biochem Biophys Res Commun. 231 (1) :56-60.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_003303.2

Q16762

Human

MVHQVLYRAL VSTKWLAESI RTGKLGPGLR VLDASWYSPG TREARKEYLE RHVPGASFFD IEECRDTASP YEMMLPSEAG FAEYVGRLGI SNHTHVVVYD GEHLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLDRS LLKTYEQVLE NLESKRFQLV DSRSQGRFLG TEPEPDAVGL DSGHIRGAVN MPFMDFLTED GFEKGPEELR ALFQTKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPESRVS QGKSEKA