Recombinant humaan RhoG-eiwit

RhoG (Ras homology Growth-related) is a member of the Rac subfamily of the Rho family of small G proteins. RhoG is a small monomeric GTP-binding protein (G protein), and is an important component of many intracellular signaling pathways. This protein are required for the formation of membrane ruffles during micropinocytosis. It plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Like most small G proteins RhoG is involved in a diverse set of cellular signaling mechanisms. In mammalian cells these include cell motility, gene transcription, endocytosis, neurite outgrowth, protection from anoikis and regulation of the neutrophil NADPH oxidase. Recombinant human RhoG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Rho-related GTP-binding protein RhoG, ARHG, Ras homolog family member G

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.2 kDa (225aa) confirmed by MALDI-TOF

Rho-related GTP-binding protein RhoG, RhoG, Rho related GTP binding protein RhoG, Ras homolog family member G, ARHG, ATGP1595-10 µg, ATGP1595-20 µg, ATGP1595-50 µg, ATGP1595-100 µg, ATGP1595-250 µg, ATGP1595-500 µg, ATGP1595-1 mg, ATGP1595-010, ATGP1595-020, ATGP1595-050, ATGP1595-100, ATGP1595-250, ATGP1595-500, ATGP1595-01M

Van Buul J.D., et al. (2007) J. Cell Biol. 178:1279-1293; ; Ellerbroek S.M., et al. (2004) Mol. Biol. Cell 15:3309-3319

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 20% glycerol, 50 mM Imidazole, 0.5M NaCl

Signal Transduction

NP_001656

P84095

Human

MQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC