Recombinant humaan RhoQ-eiwit

RHOQ, also known as rho-related GTP-binding protein RhoQ, is a small signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. It involved in epithelial cell polarization processes. It may play a role in CFTR trafficking to the plasma membrane. It causes the formation of thin, actin-rich surface projections called filopodia. Recombinant human RhoQ protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Rho-related GTP-binding protein RhoQ, ras homolog family member Q, RAS-like, family 7 member A, RASL7A, Ras-like protein TC10, ARHQ

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.7 kDa (225aa) confirmed by MALDI-TOF

RHOQ, Rho-related GTP-binding protein RhoQ precursor, Rho-related GTP-binding protein RhoQ precursor, ARHQ, RASL7A, TC10, TC10A, ATGP2081-10 µg, ATGP2081-20 µg, ATGP2081-50 µg, ATGP2081-100 µg, ATGP2081-250 µg, ATGP2081-500 µg, ATGP2081-1 mg, ATGP2081-10, ATGP2081-20, ATGP2081-50, ATGP2081-100, ATGP2081-250, ATGP2081-500, ATGP2081-1

Ridley AJ. et al. (2006) Trends Cell Biol. 16:522-529; ; Cheng J. et al. (2005) J Biol Chem. 280:3731-3739.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Signal Transduction

NP_036381

P17081

Human

MAHGPGA LMLKCVVVGD GAVGKTCLLM SYANDAFPEE YVPTVFDHYA VSVTVGGKQY LLGLYDTAGQ EDYDRLRPLS YPMTDVFLIC FSVVNPASFQ NVKEEWVPEL KEYAPNVPFL LIGTQIDLRD DPKTLARLND MKEKPICVEQ GQKLAKEIGA CCYVECSALT QKGLKTVFDE AIIAILTPKK HTVKKRIGSR CINCC