Recombinant humaan Ribokinase/RMKS-eiwit

Ribokinase, also known as RBKS, belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. Recombinant human RBKS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribokinase, RBSK

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

36.3 kDa (342aa) confirmed by MALDI-TOF

RBKS, ATGP0827-10 µg, ATGP0827-20 µg, ATGP0827-50 µg, ATGP0827-100 µg, ATGP0827-250 µg, ATGP0827-500 µg, ATGP0827-1 mg, ATGP0827-10, ATGP0827-20, ATGP0827-50, ATGP0827-100, ATGP0827-250, ATGP0827-500, ATGP0827-1

Sigrell J A., et al. (1999) J Mol Biol. 290:1009-1018.; ; Park J., et al. (2007) FEBS Lett. 581:3211-3216.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing,10% glycerol

Metabolism

NP_071411

Q9H477

Human

MAASGEPQRQ WQEEVAAVVV VGSCMTDLVS LTSRLPKTGE TIHGHKFFIG FGGKGANQCV QAARLGAMTS MVCKVGKDSF GNDYIENLKQ NDISTEFTYQ TKDAATGTAS IIVNNEGQNI IVIVAGANLL LNTEDLRAAA NVISRAKVMV CQLEITPATS LEALTMARRS GVKTLFNPAP AIADLDPQFY TLSDVFCCNE SEAEILTGLT VGSAADAGEA ALVLLKRGCQ VVIITLGAEG CVVLSQTEPE PKHIPTEKVK AVDTTGAGDS FVGALAFYLA YYPNLSLEDM LNRSNFIAAV SVQAAGTQSS YPYKKDLPLT LF