Recombinant humaan RKIP/PEBP1-eiwit

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression. Recombinant human PEBP1 was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphatidylethanolamine binding protein 1, PBP, Prostatic binding protein, RKIP, HCNP, HCNPpp, PEBP, Raf kinase inhibitory protein, Hippocampal cholinergic neurostimulating peptide

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

21 kDa (187aa) confirmed by MALDI-TOF

RKIP, PEBP1, Phosphatidylethanolamine binding protein 1, HCNP, PBP, PEBP, Phosphatidylethanolamine binding protein 1 Hippocampal cholinergic neurostimulating peptide, Neuropolypeptide h3, HCNPpp, PEBP, PEBP 1, PEBP1, Phosphatidylethanolamine binding protein, Prostatic binding protein, R kip, Raf kinase inhibitory protein RKIP., ATGP0382-10 µg, ATGP0382-20 µg, ATGP0382-50 µg, ATGP0382-100 µg, ATGP0382-250 µg, ATGP0382-500 µg, ATGP0382-1 mg, ATGP0382-10, ATGP0382-20, ATGP0382-50, ATGP0382-100, ATGP0382-250, ATGP0382-500, ATGP0382-1

Keller ET., et al. (2004) Biochem Pharmacol. 68 (6) :1049-53. ; ; Park S., et al. (2005) Oncogene. 24 (21) :3535-40. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Cell Cycle

NP_002558

P30086

Human

MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK